Enzymes used in PCR is ….? ( Botany)

NEET   Model Paper-2

Continued…  

5)choose the wrong one

a) Malacophily – Bats
b) Entomophily – Insect pollination
c) Ornithophily – Bird pollination
d) Anemophily – By wind

Ans: a

by wind, Almost all gymnosperms are anemophilous, as are many plants in the order Poales, includ -ing grasses, sedges and rushes, Entomophily insect pollination is a form of pollination whereby pollen of plants, especially but not only of flowering plants, is distributed by insects
Ornithophily or bird pollination is the pollination of flowering plants by birds Malacophily refers to when pollination of plants occurs through snails,, Chiropterophily is pollination of plants by bats

6) Enzymes used in PCR is ……?

a) Taq polymerase b) gyrase
c) transcriptase d) hexokinase

Ans: a

DNA replication in an organism, PCR requires a DNA polymerase enzyme that makes new strands of DNA, using existing strands as templates. The DNA polymer ase typically used in PCR is called Taq polymerase, after the heat-tolerant bacterium from which it was isolated (Thermus aquaticus).

7) Gel electrophoresis is used for …. .?

a) Isolation DNA molecule
b) Cutting DNA in to fragment
c) Separation of DNA fragments according to their size
d) Constraction of recombinant DNA by joining with cloning vector

Ans: c

Electrophoresis is a technique commonly used in the lab to separate charged molecules, like DNA, according to size. Gel electro phoresis is a technique commonly used in laboratories to separate charged molecules like DNA?, RNA? and proteins? according to their size.

8) PCR and Restriction fragment lenth Polymorphism are the methods for …..?

a) genetic transformation
b) DNA sequencing
c) DNA finger printing
d) Study of enzymes

Ans: b

(RFLP) PCR is a type of polymorp- hism that results from variation in the DNA sequence recognized by restriction enzymes. These are bacterial enzymes used by scientists to cut DNA molecules at known locations.
PCR and RFLP are the methods used for genetic fingerprinting. … Restriction fragment length polymorphism (RFLP) analyses the variable lengths of DNA fragments which are formed from the digestion of a DNA sample with a restriction endonuclease.

9) Agarose extracted from weeds find use in …..?

a) Spectrophotometry
b) Tissue culture
c) Gel electrophoresis
d) PCR

Ans: b

Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. Now-a-days the most commonly used matrix is agarose which is a natural polymer extracted from seaweeds (e.g., Gelidium, Gracilaria, Gigartina, etc.).

10) First hormone prepared by genetic engineering …?

a) Insulin b) oxytosin
c) adrenaline d) Somatotropin

Ans: a

Insulin is the first hormone produced by genetic engineering. It was produced by the help of E. coli. It was first marketed in the name of humulin.

11) Tissue culture technique has been biotechnologically success- ful in production of –

a) alcoholic beverages
b) cheese c) shikonin d) Insulin

Ans: c.

12) Yeast is a good source of

a) carbohydrates
b) vitamin B12
c) Proteins d) both b and c

Ans: c

Nutritional yeast is a deactivated yeast, usually S. cerevisiae. It is naturally low in fat and sodium as well as an excellent source of protein and vitamins, especially most B-complex vitamins (con-trary to some claims, it contains little or no vitamin B12 ), as well as other minerals and cofactors required for growth.

13) The micro – organism involves in making bread is …..

a) Acetobacter
b) brewer’s yeast
c) Saccharomyces cerevisiae
d) None of the above

Ans: c

Yeast is used to make bread. Yeast is a microbe which feeds on sugar. Enzymes in yeast ferment sugar forming carbon dioxide and ethanol. The carbon dioxide makes the bread rise.

14) Penicillin is obtained from –

a) Mushroom b) viruses
c) Bacteria and viruses
d) Penicillium notatum

Ans: d

The antibiotic penicillin is obtained from Penicillium notatum or P. chrysogenum (fungus).

15) A bio reactor refers to

a) Fermentation tank
b) organisms reacting to stimuli
c) Nuclear reactor for biochem- ical reactions
d) Tank & biochemical reactions

Ans: d

A bio reactor refers to any manufac- tured device or system that supports a biologically active environment. In one case, a bio reactor is a vessel in which a chemical process is carried out which involves organisms or biochemically active substances derived from such organisms.

16) Cells obtained from cancerous tumours are known as –

a) myelomas b) hybridomas
c) Lymphocytes
d) Monoclonal cells

Ans: a

Cells obtained from cancerous tumours are known as Myelomas.They are the plasma cells produced in the response to viruses, bacteria and another type of harmful substances.

17) Hybridomas are employed for

a) synthesis of antibiotics
b) Killing cancer cells
c) synthesis of monoclonal antibodies
d) Production of somatic hybrids

Ans: c

Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies). The myeloma cell line that is used in this process is selected for its ability to grow in tissue culture and for an absence of antibody synthesis.

18) Antibiotics inhibits the growth or destroy

a) Bacteria and fungi
b) Bacteria and viruses
c) Bacteria algae and viruses
d) Bacteria, fungi and viruses

Ans: d

Antibiotics are chemicals that kill or inhibit the growth of bacteria and are used to treat bacterial infections. They are produced in nature by soil bacteria and fungi.

19) Which of the following is not concerned with biotechnology ?

a) Bio gas Production
b) Sewage treatment
c) Bio fertilizers
d) Wood seasoning

Ans: d

20) which of the enzyme known as molecular Scisors …?

a) Restriction endonucleases
b) Lygases c) Topoisomerases
d) Polymerases

Ans: a

21) which of the following known as initiation codon ..?

a) UUG B) AUG
C) AGU D) UUU

Ans: b

22) Prabhavikranthi is a hybrid variety developed by IARI on whichplant ?

a) Cow pea b) Tomato
c) Okra d) Cauliflower

Ans: c

Improved varieties of chickpea, pig -eon pea and mung bean develo -ped by IARI have contributed significantly to rainfed crop production. These varieties are of short duration and most suitable for crop rotation, leading to increase in food grains production and improvement in the protein status in Indian diet

23) Which of the following is true for Golden rice?

a) It is Vitamin A enriched, with a gene from daffodil
b) It is pest resistant, with a gene from Bacillus thuringiensis
c) It is drought tolerant, developed using Agrobacterium vector
d) It has yellow grains, because of a gene introduced from a primitive variety of rice

Ans :a

Exp :Golden rice is vitamin A enric -hed rice, with agene from daffo -dil and is rich in carotene

24) Which pair is correct ?

a) – Cynobacteria – Bio pestisides
b) – Mychorriza – Absorption of phosphurus
c) – Becillus thuringiensis – toxin
d) – Single cell protien – Rhizobium .
Choose:
a) c b) a c) d d) b

Ans: a

 

25) Match the following

Column-I Column-II
(P) Aspergilus niger                (i) Butyric acid
(Q) Clostridium butiricum   (ii) Acetic acid
(R) Azatobactor                      (iii) Lactic acid
(S) Lactobacillus                     (iv) Citric acid
(a) (S-i) (P-ii) (Q-iii) (R-iv)
(b) (Q-i) (R-ii) (S-iii) (P-iv)
(c) (R-i) (P-ii) (Q-iii) (S-iv)
(d) (P-i) (Q-ii) (S-iii) (S-iv)

Ans: b

26) Choose wrong statement

a) DNA-Finger printing ___ Edwin Southern
b) PCR ___ Kary B. Mullis
c) Western blotting____by Towbin
d)Northern Blot technique ____ James Alwine, David Kemp, and George Stark at Stanford University

Ans: a

The technique of DNA finger prin- ting was developed in 1984 by British geneticist Alec Jeffreys, after he noticed that certain sequences of highly variable DNA (known as minisatellites), which do not contribute to the functions of genes, are repeated within genes
The Polymerase Chain Reaction (PCR) technique, invented in 1985 by Kary B. Mullis, allowed scientists to make millions of copies of a scarce sample of DNA
Western blotting also known as immuno blotting or protein blotting, introduced by Towbin etal., is a commonly used technique for the immune detection and quantification of proteins in vitro
The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University, with contr- ibutions from Gerhard Heinrich. Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern.